anti mil 21 antibody (R&D Systems)
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anti mil 21 antibody
Anti Mil 21 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+il+21/Mouse+IL-21+Antibody/pmc12827887-70-12-15
Average 92 stars, based on 7 article reviews
Anti Mil 21 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+il+21/Mouse+IL-21+Antibody/pmc12827887-70-12-15
Average 92 stars, based on 7 article reviews
anti mil 21 antibody - by Bioz Stars,
2026-09
92/100 stars
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Western Blot:Article Title: Interleukin-6/interleukin-21 signaling axis is critical in the pathogenesis of pulmonary arterial hypertension Article Snippet: Frozen lungs were homogenized in 50 mM Hepes, 100 mM sodium fluoride, 2 mM sodium orthovanadate, 4 mM EDTA, 1% Tween-20, 0.1% SDS, and Complete protease inhibitor mixture (Roche Applied Science) using a Polytron homogenizer (PT 10-35GT; Kinematica) as described previously ( 44 ). .. The precleared lysates then were resolved by SDS/PAGE and subjected to Western blot analysis using a standard procedure with the following antibodies: anti–pSTAT3-Tyr705 (clone D3A7; Cell Signaling), anti-STAT3 (clone 79D7; Cell Signaling), anti-RELM alpha (Fizz1) (ab39626; Abcam), anti–IL-17A (sc-52567; Santa Cruz), and Article Title: The signaling suppressor CIS controls proallergic T cell development and allergic airway inflammation Article Snippet: .. Immunoblot analysis Total CD4 + T cells sorted by magnetic-activated cell sorting were activated for various times by plate-bound anti-CD3 (2C11; BioXCell) and anti-CD28 (37.51; BioXCell) in the presence or absence of IL-2 or anti-IL-4 (11B11; BioXCell) or anti-IL-6 (MP520F3; R&D Systems) or/and In Vitro:Article Title: Retinoic Acid Hypersensitivity Promotes Peripheral Tolerance in Recent Thymic Emigrants Article Snippet: Some cultures were also treated with at-RA (Sigma) at the indicated concentrations. .. For in vitro cytokine neutralization, anti-IFN-γ (clone XMG1.2, UAB Hybridoma Center), Neutralization:Article Title: Retinoic Acid Hypersensitivity Promotes Peripheral Tolerance in Recent Thymic Emigrants Article Snippet: Some cultures were also treated with at-RA (Sigma) at the indicated concentrations. .. For in vitro cytokine neutralization, anti-IFN-γ (clone XMG1.2, UAB Hybridoma Center), Control:Article Title: Retinoic Acid Hypersensitivity Promotes Peripheral Tolerance in Recent Thymic Emigrants Article Snippet: Some cultures were also treated with at-RA (Sigma) at the indicated concentrations. .. For in vitro cytokine neutralization, anti-IFN-γ (clone XMG1.2, UAB Hybridoma Center), Concentration Assay:Article Title: Retinoic Acid Hypersensitivity Promotes Peripheral Tolerance in Recent Thymic Emigrants Article Snippet: Some cultures were also treated with at-RA (Sigma) at the indicated concentrations. .. For in vitro cytokine neutralization, anti-IFN-γ (clone XMG1.2, UAB Hybridoma Center), Incubation:Article Title: Digoxin ameliorates autoimmune arthritis via suppression of Th17 differentiation. Article Snippet: Contents lists available at ScienceDirect International Immunopharmacology j ourna l homepage: www.e lsev ie r .com/ locate / in t imp Digoxin ameliorates autoimmune arthritis via suppression of Th17 differentiation Jennifer Lee a,b, Seungye Baek a, Jaeseon Lee a, Juhyun Lee a, Dong-Gun Lee a, Mi-Kyung Park a, Mi-La Cho a, Sung-Hwan Park a,b, Seung-Ki Kwok a,b,⁎ a The Rheumatism Research Center, Catholic Research Institute of Medical Science, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul 137-701, Republic of Korea b Division of Rheumatology, Department of InternalMedicine, Seoul St.Mary's Hospital, College ofMedicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul 137-701, Republic of Korea ⁎ Corresponding author at: Division of Rheumatology, D Seoul St. Mary's Hospital, College of Medicine, The Cat Banpo-daero, Seocho-gu, Seoul 137-701, Republic of K fax: +82 2 599 3589.. E-mail address: seungki73@catholic.ac.kr (S.-K. Kwok) http://dx.doi.org/10.1016/j.intimp.2015.03.017 1567-5769/© 2015 Elsevier B.V. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 1 September 2014 Received in revised form 2 March 2015 Accepted 13 March 2015 Available online 25 March 2015 Keywords: Digoxin ROR γt Rheumatoid arthritis IL-17 producing T cells Regulatory T cells Digoxin is a cardiac glycoside that is commonly used to treat heart failure.. Based on its known anti-inflammatory effect, this study was undertaken to investigate the effect of digoxin on collagen-induced arthritis (CIA) and to delineate the underlying mechanism. FACS:Article Title: The signaling suppressor CIS controls proallergic T cell development and allergic airway inflammation Article Snippet: .. Total CD4 + T cells sorted by magnetic-activated cell sorting were activated for various times by plate-bound anti-CD3 (2C11; BioXCell) and anti-CD28 (37.51; BioXCell) in the presence or absence of IL-2 or anti-IL-4 (11B11; BioXCell) or anti-IL-6 (MP520F3; R&D Systems) or/and Article Title: The signaling suppressor CIS controls proallergic T cell development and allergic airway inflammation Article Snippet: .. Immunoblot analysis Total CD4 + T cells sorted by magnetic-activated cell sorting were activated for various times by plate-bound anti-CD3 (2C11; BioXCell) and anti-CD28 (37.51; BioXCell) in the presence or absence of IL-2 or anti-IL-4 (11B11; BioXCell) or anti-IL-6 (MP520F3; R&D Systems) or/and Staining:Article Title: Aging promotes reorganization of the CD4 T cell landscape toward extreme regulatory and effector phenotypes Article Snippet: The following antibodies were used for membranal staining: PE-conjugated anti-CTLA4 (4C10-4B9; BioLegend), PE/cy7-conjugated anti-CD25 (3C7; BioLegend), AF700-conjugated anti-CD62L (Mel-14; BioLegend), BV605- or BV785-conjugated anti-PD1 (29F.1A12; BioLegend), APC-conjugated anti-CD81 (Eat-2; BioLegend), FITC-conjugated anti-CD8 (53-6.7; BioLegend), PerCP/cy5.5-conjugated anti-CD44 (IM7; BioLegend), AF700- or BV785-conjugated anti-CD4 (RM4-5; BioLegend), PE-conjugated anti-CD121b (4E2; BD Biosciences), BV421-conjugated anti-CD25 (PC61; BioLegend), BV605-conjugated anti-CD195 (C34-3448; BD Biosciences), BV785-conjugated anti-LAG3 (C9B7W; BioLegend), BV421-conjugated anti-CD4 (GK1.5; BioLegend), PerCP/cy5.5-conjugated anti-CD8 (53-6.7; BioLegend), PE-conjugated anti-CD137 (17B5; BioLegend), PE/cy7-conjugated anti-CD134 (OX-86; BioLegend), and PE-conjugated anti-CD178 (MFL3; eBioscience). .. After staining for membranal markers, intracellular labeling was performed: Cells were fixed and permeabilized using the FOXP3/Transcription Factor Staining Kit (eBioscience), blocked with Rat serum (1 μl per 100 μl of staining buffer), and stained with the following antibodies: BV605-conjugated anti-TNF (MP6-XT22; BioLegend), BV605-conjugated anti–IL-17a (TC11-18H10.1; BioLegend), FITC- or BV510-conjugated anti–IL-2 (JES6-5H4; BioLegend), BV421- or BV786-conjugated anti-IFNγ (XMG1.2; BioLegend), BV421-conjugated anti–IL-10 (JES5-16E3; BioLegend), APC-conjugated anti-GzmB (QA16A02; BioLegend), PE-conjugated anti-CCL5 (2E9/CCL5; BioLegend), PE/cy7-conjugated anti-EOMES (Dan11mag; eBioscience), AF488-conjugated anti-FOXP3 (150D; BioLegend), APC-conjugated Article Title: TRIM14 inhibits OPTN-mediated autophagic degradation of KDM4D to epigenetically regulate inflammation Article Snippet: .. Cells were stained with the following fluorescence-labeled antibodies: APC-Cy7–conjugated anti-CD45 (BD, B557659), PE-ef610–conjugated CD4 (eBioscience, 61004382), BV785-conjugated anti-CD8 (eBioscience, 60-5961), BV510-conjugated anti–IL-17A (BD, 563295) and PE-conjugated Labeling:Article Title: Aging promotes reorganization of the CD4 T cell landscape toward extreme regulatory and effector phenotypes Article Snippet: The following antibodies were used for membranal staining: PE-conjugated anti-CTLA4 (4C10-4B9; BioLegend), PE/cy7-conjugated anti-CD25 (3C7; BioLegend), AF700-conjugated anti-CD62L (Mel-14; BioLegend), BV605- or BV785-conjugated anti-PD1 (29F.1A12; BioLegend), APC-conjugated anti-CD81 (Eat-2; BioLegend), FITC-conjugated anti-CD8 (53-6.7; BioLegend), PerCP/cy5.5-conjugated anti-CD44 (IM7; BioLegend), AF700- or BV785-conjugated anti-CD4 (RM4-5; BioLegend), PE-conjugated anti-CD121b (4E2; BD Biosciences), BV421-conjugated anti-CD25 (PC61; BioLegend), BV605-conjugated anti-CD195 (C34-3448; BD Biosciences), BV785-conjugated anti-LAG3 (C9B7W; BioLegend), BV421-conjugated anti-CD4 (GK1.5; BioLegend), PerCP/cy5.5-conjugated anti-CD8 (53-6.7; BioLegend), PE-conjugated anti-CD137 (17B5; BioLegend), PE/cy7-conjugated anti-CD134 (OX-86; BioLegend), and PE-conjugated anti-CD178 (MFL3; eBioscience). .. After staining for membranal markers, intracellular labeling was performed: Cells were fixed and permeabilized using the FOXP3/Transcription Factor Staining Kit (eBioscience), blocked with Rat serum (1 μl per 100 μl of staining buffer), and stained with the following antibodies: BV605-conjugated anti-TNF (MP6-XT22; BioLegend), BV605-conjugated anti–IL-17a (TC11-18H10.1; BioLegend), FITC- or BV510-conjugated anti–IL-2 (JES6-5H4; BioLegend), BV421- or BV786-conjugated anti-IFNγ (XMG1.2; BioLegend), BV421-conjugated anti–IL-10 (JES5-16E3; BioLegend), APC-conjugated anti-GzmB (QA16A02; BioLegend), PE-conjugated anti-CCL5 (2E9/CCL5; BioLegend), PE/cy7-conjugated anti-EOMES (Dan11mag; eBioscience), AF488-conjugated anti-FOXP3 (150D; BioLegend), APC-conjugated Fluorescence:Article Title: TRIM14 inhibits OPTN-mediated autophagic degradation of KDM4D to epigenetically regulate inflammation Article Snippet: .. Cells were stained with the following fluorescence-labeled antibodies: APC-Cy7–conjugated anti-CD45 (BD, B557659), PE-ef610–conjugated CD4 (eBioscience, 61004382), BV785-conjugated anti-CD8 (eBioscience, 60-5961), BV510-conjugated anti–IL-17A (BD, 563295) and PE-conjugated |
